Akatsuki (; Literally meaning Dawn or Daybreak) is a criminal organization of S-Clas* missing-nins and is the most wanted group in all of shinobi world. Their main goal is to collect all of the tailed beasts for their plan of world domination. As such, Akatsuki, despite initially having a minor role during Part I, have become the primary antagonists in Part II.

Akatsuki at any given time is composed of no more than ten primary members, all of whom were S-Clas* criminals of their own respective villages. Members always work in teams of two with the exception of Zetsu, who functions as the organization's spy, and make use of their unique skills to the team's advantage. Team members must function very well or at least to accomplish their task even though they have a mutual problems with each other. The organization is rarely fully as*embled, as seen in the end of Part I, when one member said it was the first time in seven years when they were all together.

Akatsuki is widespread, spanning several countries with agents in various places. When one team finds a tailed beast and has to begin the job of sealing the beast, they have hidden hideouts with many traps ready, to begin the sealing with the organization's leader's astral projection for a three-day long jutsu.